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immpress horseradish peroxidase horse anti rabbit igg secondary antibody  (Vector Laboratories)


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    Vector Laboratories immpress horseradish peroxidase horse anti rabbit igg secondary antibody
    Immpress Horseradish Peroxidase Horse Anti Rabbit Igg Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 335 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/horse+anti+rabbit+secondary+antibody/ImmPRESS+Horse+Anti-Rabbit+IgG+PLUS+Polymer+Kit%2C+Peroxidase/10__34067_slash_kid__0000001149-118-23-32
    Average 96 stars, based on 335 article reviews
    immpress horseradish peroxidase horse anti rabbit igg secondary antibody - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Staining:

    Article Title: A single local delivery of paclitaxel and nucleic acids via an immunoactive polymer eliminates tumors and induces antitumor immunity
    Article Snippet: .. Heart, liver, spleen, lung and kidney sections were stained with H&E, and tumor sections were stained with rat anti-mouse CD8a monoclonal antibody (eBioscience, clone 4SM15) followed by goat anti-rat secondary antibody (Vector Labs, MP-5444) or with rabbit anti-mouse PD-L1 antibody (Novus biologicals, clone 2096A) followed by horse anti-rabbit secondary antibody (Vector Labs, MP-5401). .. Slides were rinsed twice in tris buffered saline with Tween® 20, applied with vector immPACT red (Vector Labs, SK-5105) for 20 min and transferred to a Leica Autostainer XL for hematoxylin counterstain, dehydration and coverslipping.

    Incubation:

    Article Title: Neural correlates of adversity-overcoming pup rescue behavior in female mice.
    Article Snippet: The sections were washed with PBS containing 0.2% Triton-100 (PBST), incubated with 0.3% H2O2 in methanol for 5 minutes, washed with PBST, blocked with 0.8% Block Ace (Dainihon-Seiyaku, Osaka, Japan) in PBST, and incubated at 4°C overnight with rabbit primary antibody against c-Fos (1:5000, Cat# sc-52, RRID: AB_2106783, Santa Cruz Biotechnology, Inc., Dallas, TX, USA). .. The following morning, the sections were washed and incubated with biotinconjugated horse anti-rabbit secondary antibody (1:2000, Cat# BA-1100, RRID: AB_2336201, Vector Laboratories, Inc., Burlingame, CA, USA) for 2 hours and then in ABC peroxidase reagent (Cat# PK6100; Vectastain ABC Elite kit; Vector Laboratories) for 1 hour according to the manufacturer’s instructions. .. The labeling was visualized by incubation in 3,30’–diaminobenzidine (DAB) solution with nickel intensification (DAB peroxidase substrate kit Cat#SK-4100, Vector Laboratories) for 5 minutes.

    Article Title: Deprivation of EGFR signal causes senolysis in PDAC with CDK4/6 inhibition.
    Article Snippet: Sections were deparaffinized, hydrated, and underwent heatinduced antigen retrieval, followed by incubation with primary antibody anti-RFP (PM005, MBL, RRID: AB_591279) at 4°C overnight. .. All tissues except the intestine samples were washed and incubated with HRPconjugated horse anti-rabbit secondary antibody (MP-7401, VectorLabs, RRID: AB_2336529). .. Fluorescent signals were amplified using the TSA Plus Fluorescein (Akoya Biosciences).

    Article Title: Oleic acid from cancer-associated fibroblast promotes cancer cell stemness by stearoyl-CoA desaturase under glucose-deficient condition
    Article Snippet: IHC for SCD was performed in lung adenocarcinoma tissue microarray (US Biomax, Rockville, MD, USA) according to the manufacturer’s instructions [ ]. .. The prepared tissues microarray slides were incubated with SCD antibody (Cell-Signaling technology) for overnight and incubated with horse anti-rabbit secondary antibody (Vector Laboratories, Burlingame, CA, USA) for 2 h. Following treatment with 3,3’-diaminobenzidine solution (Vector Laboratories), slides were counterstained using hematoxylin, dehydrated in alcohol, and mounted in a xylene-based solution. .. The mean IHC staining intensity for SCD was quantified using ImageJ software and scored 0–3 as follows [ ]: 0, no staining; 1, < 15 mean intensity; 2, 15–50 mean intensity; and 3, > 50 mean intensity.

    Article Title: State-dependent alteration of respiration in a rat model of Parkinson disease
    Article Snippet: Sections were incubated overnight at room temperature in a primary rabbit tyrosine hydroxylase antibody (1/300; P40101, Pel-Freez). .. After incubation for 120min at room temperature in a horse anti-rabbit secondary antibody (1/200; BA-1100, Vector laboratories), sections were processed with avidin-biotin-peroxidase complex (1/200 ; Vectastain Elite ABC-HRP kit, Eurobio) for 30 min. .. Finally, peroxidase detection was conducted with 0.066% of 3,3-diaminobenzidine-tetrahydrochloride (DAB; Sigma-Aldrich), 0.03% NiCl 2 and 0.06% H 2 O 2 .

    Article Title: Oleic acid from cancer-associated fibroblast promotes cancer cell stemness by stearoyl-CoA desaturase under glucose-deficient condition.
    Article Snippet: Analysis of SCD expression in lung adenocarcinoma tissue microarray IHC for SCD was performed in lung adenocarcinoma tissue microarray (US Biomax, Rockville, MD, USA) according to the manufacturer’s instructions [37]. .. The prepared tissues microarray slides were incubated with SCD antibody (Cell-Signaling technology) for overnight and incubated with horse anti-rabbit secondary antibody (Vector Laboratories, Burlingame, CA, USA) for 2 h. Following treatment with 3,3’-diaminobenzidine solution (Vector Laboratories), slides were counterstained using hematoxylin, dehydrated in alcohol, and mounted in a xylene-based solution. .. The mean IHC staining intensity for SCD was quantified using ImageJ software and scored 0–3 as follows [38]: 0, no staining; 1, < 15 mean intensity; 2, 15–50 mean intensity; and 3, > 50 mean intensity.

    Article Title: A calcitonin receptor-expressing subregion of the medial preoptic area is involved in alloparental tolerance in common marmosets.
    Article Snippet: The sections were incubated at 4 °C three overnight with rabbit primary antibodies against Calcr (1:250, AHP635, BioRad) or c-Fos (1:5000, sc-52, Santa Cruz Biotechnology, Inc., Dallas, TX, USA) (note: the staining results were notably varied by the c-Fos antibody lot numbers). .. The following morning the sections were washed and incubated with biotinconjugated horse anti-rabbit secondary antibody (1:2000, BA-1100, Vector Laboratories, Inc., Burlingame, CA, USA) for 2 h and then in ABC peroxidase reagent (Vectastain ABC Elite kit; Vector Laboratories) for 1 h according to the manufacturer’s instructions. ..

    Article Title: State-dependent alteration of respiration in a rat model of Parkinson's disease.
    Article Snippet: Sections were incubated overnight at room temperature in a primary rabbit tyrosine hydroxylase antibody (1/300; P40101, Pel-Freez). .. After incubation for 120 min at room temperature in a horse anti-rabbit secondary antibody (1/200; BA-1100, Vector laboratories), sections were processed with avidin-biotin-peroxidase complex (1/200; Vectastain Elite ABC-HRP kit, Eurobio) for 30 min. .. Finally, peroxidase detection was conducted with 0.066% of 3,3-diaminobenzidine-tetrahydrochloride (DAB; Sigma-Aldrich), 0.03% NiCl2 and 0.06% H2O2.

    Microarray:

    Article Title: Oleic acid from cancer-associated fibroblast promotes cancer cell stemness by stearoyl-CoA desaturase under glucose-deficient condition
    Article Snippet: IHC for SCD was performed in lung adenocarcinoma tissue microarray (US Biomax, Rockville, MD, USA) according to the manufacturer’s instructions [ ]. .. The prepared tissues microarray slides were incubated with SCD antibody (Cell-Signaling technology) for overnight and incubated with horse anti-rabbit secondary antibody (Vector Laboratories, Burlingame, CA, USA) for 2 h. Following treatment with 3,3’-diaminobenzidine solution (Vector Laboratories), slides were counterstained using hematoxylin, dehydrated in alcohol, and mounted in a xylene-based solution. .. The mean IHC staining intensity for SCD was quantified using ImageJ software and scored 0–3 as follows [ ]: 0, no staining; 1, < 15 mean intensity; 2, 15–50 mean intensity; and 3, > 50 mean intensity.

    Article Title: Oleic acid from cancer-associated fibroblast promotes cancer cell stemness by stearoyl-CoA desaturase under glucose-deficient condition.
    Article Snippet: Analysis of SCD expression in lung adenocarcinoma tissue microarray IHC for SCD was performed in lung adenocarcinoma tissue microarray (US Biomax, Rockville, MD, USA) according to the manufacturer’s instructions [37]. .. The prepared tissues microarray slides were incubated with SCD antibody (Cell-Signaling technology) for overnight and incubated with horse anti-rabbit secondary antibody (Vector Laboratories, Burlingame, CA, USA) for 2 h. Following treatment with 3,3’-diaminobenzidine solution (Vector Laboratories), slides were counterstained using hematoxylin, dehydrated in alcohol, and mounted in a xylene-based solution. .. The mean IHC staining intensity for SCD was quantified using ImageJ software and scored 0–3 as follows [38]: 0, no staining; 1, < 15 mean intensity; 2, 15–50 mean intensity; and 3, > 50 mean intensity.

    Avidin-Biotin Assay:

    Article Title: State-dependent alteration of respiration in a rat model of Parkinson disease
    Article Snippet: Sections were incubated overnight at room temperature in a primary rabbit tyrosine hydroxylase antibody (1/300; P40101, Pel-Freez). .. After incubation for 120min at room temperature in a horse anti-rabbit secondary antibody (1/200; BA-1100, Vector laboratories), sections were processed with avidin-biotin-peroxidase complex (1/200 ; Vectastain Elite ABC-HRP kit, Eurobio) for 30 min. .. Finally, peroxidase detection was conducted with 0.066% of 3,3-diaminobenzidine-tetrahydrochloride (DAB; Sigma-Aldrich), 0.03% NiCl 2 and 0.06% H 2 O 2 .

    Article Title: State-dependent alteration of respiration in a rat model of Parkinson's disease.
    Article Snippet: Sections were incubated overnight at room temperature in a primary rabbit tyrosine hydroxylase antibody (1/300; P40101, Pel-Freez). .. After incubation for 120 min at room temperature in a horse anti-rabbit secondary antibody (1/200; BA-1100, Vector laboratories), sections were processed with avidin-biotin-peroxidase complex (1/200; Vectastain Elite ABC-HRP kit, Eurobio) for 30 min. .. Finally, peroxidase detection was conducted with 0.066% of 3,3-diaminobenzidine-tetrahydrochloride (DAB; Sigma-Aldrich), 0.03% NiCl2 and 0.06% H2O2.



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